Technology development to identify disease specific immunoglobulins
One of our goal in the research is to identify and validate a panel of biomarkers in form of immunoglobulin, especially IgG for various diseases, including sarcoidosis and tuberculosis. Additionally, we aim to identify the clinical values of identified IgG in predicting stages and outcome of sarcoidosis. Our lab developed a novel antigen library derived from human lung cells supported by various grants by the National Heart, Lungs, and Blood Institute (NHLBI). For this purpose, we constructed a T7 phage cDNA library of potential sarcoidosis antigens using mRNA isolated from BALs and WBCs of sarcoid patients. This cDNA library was immunoscreened with sera from patients with sarcoidosis and healthy subjects and patients with tuberculosis (TB). Additionally, we used sera from smear positive TB subjects and as negative controls we used sera from healthy subjects, cystic fibrosis and lung cancer. We identified biomarkers with high specificity and sensitivity which can classify TB from cystic fibrosis.
Related publications:
1. Samer Najeeb Hanoudi, Harvinder Talwar, Sorin Draghici and Lobelia Samavati. Autoantibodies against cytoskeletons and lysosomal trafficking discriminate sarcoidosis from healthy controls, tuberculosis and lung cancers. Molecular Biomedicine (2022-01-20).
2. Talwar H, Hanoudi SN, Draghici S and Samavati L. Novel T7 Phage Display Library Detects Classifiers for Active Mycobacterium Tuberculosis Infection. Viruses. 2018 Jul 19;10(7). pii: E375. doi: 10.3390/v10070375.
3. Harvinder Talwar, Samer Najeeb Hanoudi, Andreea Geamanu*, Dana Kissner, Sorin Draghici and L. Samavati. Detection of Cystic Fibrosis Serological Biomarkers Using a T7 Phage Display Library. Scientific Reports (nature publishing group). Sci Rep. Dec 18;7(1):17745. doi: 10.1038/s41598-017-18041-
4.Harvinder Talwar, Jaya Talreja* and Lobelia Samavati. T7 Phage Display Library a Promising Strategy to Detect Tuberculosis Specific Biomarkers. Mycobact Dis. 2016 Jun;6(2). pii: 214. Epub 2016 Jun 28. PMID: 27867751.
5. Harvinder Talwar, Rita Rosati, Jia Li, Dana Kissner, Samiran Ghosh, Félix Fernández -Madrid and Lobelia Samavati. Development of a T7 Phage Display Library to Detect Sarcoidosis and Tuberculosis by a Panel of Novel Antigens. Ebiomedicine. April 2015. Pages 341–350.